Article
Digitally encoded silica microparticles for multiplexed nucleic acid detection.
Chemical communications (Cambridge, England) - 30 May 2017
Xu Weiwei, Chen Chao, Ma Xiaodong, Yuan Lihua, Liu Shenquan, Zheng Kexiao, Li Jiong
Abstract excerpt
A robust high throughput suspension array was proposed to analyze biomolecules. To exploit its capacity for nucleic acid detection, we designed a multiplex nested asymmetric PCR (MNAS PCR) that can produce single stranded DNA (ssDNA) efficiently without complicated optimization. Multiplexed HPV genotyping was demonstrated with high selectivity, high sensitivity and rapid hybridization within 20 minutes.
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