Article
Deep profiling of human T cells defines compartmentalized clones and phenotypic trajectories across blood and tonsils.
Immunity - 9 Dec 2025
Sureshchandra Suhas, Henderson James, Levendosky Elizabeth, Bhattacharyya Sankalan, Kastenschmidt Jenna M, Sorn Andrew M, Mitul Mahina Tabassum, Yates Timothy B, Cheng Evien, Benchorin Aviv, Batucal Kyle, Daugherty Allyssa, Murphy Samuel J H, Thakur Chandrani, Trask Douglas, Ahuja Gurpreet, Zhong Qiu, Moisan Annie, Tiffeau-Mayer Andreas, Saligrama Naresha, Wagar Lisa E
Abstract excerpt
98% of T cells reside in tissues, yet nearly all human T cell analyses are performed on peripheral blood. We performed single-cell sequencing of 5.7 million T cells from autologous blood and tonsils of ten donors. We identified distinct patterns of clonal expansion associated with tonsil-restricted phenotypes. Clonal sharing between blood and tonsils was lower than previous estimates and increased with age....
Read the complete abstract on PubMedTopics
- Humans
- Palatine Tonsil
- Receptors, Antigen, T-Cell
- T-Lymphocytes