Deciphering mutational effects on inducible NO synthase conformational dynamics via quantitative cross-linking mass spectrometry and AlphaFold2 subsampling.

Abstract excerpt

Mammalian nitric oxide synthases (NOSs) are flavo-hemoproteins that rely on dynamic interdomain interactions for activity. Calmodulin (CaM) facilitates specific, interdomain FMN-heme interactions that enable inter-subunit FMN-heme electron transfer essential for nitric oxide biosynthesis. Our quantitative cross-linking mass spectrometry (qXL MS) results demonstrate that the abundance of intersubunit cross-links...

Topics

Join the communities discussing this publication.

• Calmodulin
• Cross-Linking Reagents
• Flavin Mononucleotide
• Heme
• Mass Spectrometry