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CRDSAT under Heat: Balancing Stability, Affinity, and Functional Utility of Computationally Designed Tag Variants.

Biochemistry - 2 Sept 2025

Guiot Esteban, Chagot Marie-Eve, Boutilliat Alexis, Reboul Pascal, Kriznik Alexandre, Quinternet Marc

Abstract excerpt

In general, the easier and cheaper the expression and purification processes are, the more profitable the production of a recombinant protein of interest is, especially in the industrial world. Previously, we have developed the lectinic CRDSAT tag that we demonstrated is efficient at cost-effectively purifying passenger proteins. It also has the advantage of being quite small and limiting steric hindrance upon...

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PMID: 40610384
DOI: 10.1021/acs.biochem.5c00128

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