Article
Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains
26 Jan 2007
Abstract excerpt
BACKGROUND: Recombinant antibodies from Camelidae (VHHs) are potentially useful tools for both basic research and biotechnological applications because of their small size, robustness, easy handling and possibility to refold after chemio-physical denaturation. Their heat tolerance is a particularly interesting feature because it has been recently related to both high yields during recombinant expression and...
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