Direct single cell-type gene expression analysis in peripheral blood: novel ratio-based gene expression biomarkers using 2 novel monocyte reference genes (PSAP and CTSS) for detection of bacterial infection

Abstract excerpt

BACKGROUND: To determine single-cell-type gene expression in peripheral blood (PB) requires either prior cell sorting or single-cell RNA sequencing. We developed a novel ratio-based biomarker (RBB) called Direct Leukocyte Subpopulation-Transcript Abundance (DIRECT LS-TA) that allows quantification of monocyte-specific gene expression directly from PB without cell sorting. METHODS: DIRECT LS-TA leverages...

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