Egress-enhancing mutation reveals the inefficiency of non-enveloped virus cell exit.

Abstract excerpt

Viruses encounter a range of selective pressures, but inefficiencies during replication can be masked. To uncover factors that limit viral replication, we used forward genetics to enrich for a murine norovirus (MNV) mutant with faster replication. We sequentially harvested the earliest progeny in cultured cells and identified a single amino acid change in the viral NS3 protein, K40R, that was sufficient to...

Topics

• Animals
• Norovirus
• Mice
• Virus Replication
• Mutation
• Viral Nonstructural Proteins
• Caliciviridae Infections