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Article

High-dimensional temporal mapping of CAR T cells reveals phenotypic and functional remodeling during manufacturing.

Molecular therapy : the journal of the American Society of Gene Therapy - 7 May 2025

Cadinanos-Garai Amaia, Flugel Christian L, Cheung Anson, Jiang Enzi, Vaissié Alix, Abou-El-Enein Mohamed

Abstract excerpt

Despite the notable success of chimeric antigen receptor (CAR) T cell therapies in hematological malignancies, clinical outcomes remain variable, making it critical to understand how manufacturing influences product composition and function. We developed a 36-marker spectral flow cytometry panel enabling integrated profiling of phenotypic, metabolic, and functional attributes across CAR T cell production....

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Identifiers

PMID: 40315840
DOI: 10.1016/j.ymthe.2025.04.006
PMCID: PMC12126796

Genes

CD4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

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