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Article

Development of a 3-day manufacturing method to generate CD19-CD20-CD22 trispecific CAR T-cells from whole blood.

Journal of translational medicine - 15 Jul 2026

Vignola Isabella, Prochazkova Michaela, Shao Lipei, Fuksenko Tatyana, Sarkar Sarmila, Lei Hong, Jin Jianjian, Xiong Ying, Xie Yanping, Oparaocha Ibeawuchi, Slessareva Oxana, Forrest Megan, Orentas Rimas, Zhu Zhongyu, Dropulic Boro, Jin Ping, Somerville Robert P, Stroncek David F, Song Hannah W, Highfill Steven L

Abstract excerpt

BACKGROUND: Chimeric antigen receptor (CAR) T-cell therapy has transformed the treatment landscape for many hematological malignancies. However, high relapse rates and limited accessibility remain significant challenges. We developed a 3-day streamlined process to address these limitations. METHODS: T-cells were isolated from whole blood collected from healthy donors via an automated density gradient separation...

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Share to Antigens, CD19
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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 42458494
DOI: 10.1186/s12967-026-08306-8
PMCID: PMC13378159

Genes

CD19
CD22

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 18 Oct 2026.

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