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Article

CRISPR/Cas9 mediated generation of zebrafish f9a mutant as a model for hemophilia B.

Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis - 1 Apr 2025

Dhinoja Sanchi, Qaryoute Ayah Al, Deebani Afnan, De Maria Anthony, Jagadeeswaran Pudur

Abstract excerpt

AIM: This study aimed to develop a zebrafish model for hemophilia B by creating a f9a knockout, as f9a has previously demonstrated functional similarity to human Factor IX. METHODS: Using CRISPR/Cas9 technology, two gRNAs targeting exon 8 of the f9a gene, were injected along with Cas9 protein into single-cell zebrafish wild-type embryos. DNA was harvested from the tail tips of the resulting adult zebrafish and...

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Identifiers

PMID: 40127118
DOI: 10.1097/MBC.0000000000001355
PMCID: PMC11970983

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