A collagen IV fluorophore knock-in toolkit reveals trimer diversity in C. elegans basement membranes.

doi:10.1083/jcb.202412118

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Article

A collagen IV fluorophore knock-in toolkit reveals trimer diversity in C. elegans basement membranes.

The Journal of cell biology - 2 Jun 2025

Srinivasan Sandhya, Ramos-Lewis William, Morais Mychel R P T, Chi Qiuyi, Soh Adam W J, Williams Emily, Lennon Rachel, Sherwood David R

Abstract excerpt

The type IV collagen triple helix, composed of three ⍺-chains, is a core basement membrane (BM) component that assembles into a network within BMs. Endogenous tagging of all ⍺-chains with genetically encoded fluorophores has remained elusive, limiting our understanding of this crucial BM component. Through genome editing, we show that the C termini of the C. elegans type IV collagen ⍺-chains EMB-9 and LET-2 can...

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PMID: 40100062
DOI: 10.1083/jcb.202412118
PMCID: PMC11917169

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