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Article

Engineering Base Changes and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease.

Current protocols - 1 Feb 2025

Gertsenstein Marina, Lintott Lauri G, Nutter Lauryl M J

Abstract excerpt

Mice carrying patient-associated base changes are powerful tools to define the causality of single-nucleotide variants to disease states. Epitope tags enable immuno-based studies of genes for which no antibodies are available. These alleles enable detailed and precise developmental, mechanistic, and translational research. The first step in generating these alleles is to identify within the target sequence-the...

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Identifiers

PMID: 39999224
DOI: 10.1002/cpz1.70109
PMCID: PMC11856344

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