Stabilizing Mutations Enhance Evolvability of BlaC β-lactamase by Widening the Mutational Landscape.

Abstract excerpt

Antimicrobial resistance is fueled by the rapid evolution of β-lactamases. However, a gain of new enzyme activity often comes at the expense of reduced protein stability. This evolutionary constraint is often overcome by the acquisition of stabilizing mutations that compensate for the loss of stability invoked by new function mutations. Here, we report three stabilizing mutations (I105F, H184R, and V263I) in...

Topics

• beta-Lactamases
• Escherichia coli
• Mycobacterium tuberculosis
• Mutation
• Evolution, Molecular
• Enzyme Stability