Article
Development of a vitrified CRISPR/Cas12b-based assay for rapid genotyping of SLCO1B1 SNPs without DNA amplification.
Analytical methods : advancing methods and applications - 27 Feb 2025
Lu Chen, Chen Jiayue, Zhang Shengsheng, Tian Yaru, Ying Xinyu, Zhang Dan, Luo Zhidan, Si Xinxin, Li Ming
Abstract excerpt
Two single nucleotide polymorphisms (SNPs) in the human SLCO1B1 gene, c.388A>G (rs2306283) and c.521T>C (rs4149056), are independent determinants of the efficacy and side effects of statin drugs. Multinational clinical guidelines recommend testing for SLCO1B1 genotypes before the initial use of statins. Current SLCO1B1 SNP identification methods, primarily based on quantitative fluorescence PCR, rely on expensive...
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