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Rapid Generation of Reverse Genetics Systems for Coronavirus Research and High‐Throughput Antiviral Screening Using Gibson DNA Assembly

1 Jan 2025

Abstract excerpt

Coronaviruses (CoVs) pose a significant threat to human health, as demonstrated by the COVID-19 pandemic. The large size of the CoV genome (around 30 kb) represents a major obstacle to the development of reverse genetics systems, which are invaluable for basic research and antiviral drug screening. In this study, we established a rapid and convenient method for generating reverse genetic systems for various CoVs...

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PMID: 39821287
DOI: 10.1002/jmv.70171

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