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Article

Systematic assembly of a full-length infectious cDNA of mouse hepatitis virus strain A59.

Journal of virology - 1 Nov 2002

Yount Boyd, Denison Mark R, Weiss Susan R, Baric Ralph S

Abstract excerpt

A novel method was developed to assemble a full-length infectious cDNA of the group II coronavirus mouse hepatitis virus strain A59 (MHV-A59). Seven contiguous cDNA clones that spanned the 31.5-kb MHV genome were isolated. The ends of the cDNAs were engineered with unique junctions and assembled with only the adjacent cDNA subclones, resulting in an intact MHV-A59 cDNA construct of approximately 31.5 kb in...

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Identifiers

PMID: 12368349
DOI: 10.1128/jvi.76.21.11065-11078.2002
PMCID: PMC136593

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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