Construction and modification of a low-copy plasmid-based infectious clone for GI-19 genotype IBV via Red/ET recombineering: A simplified and efficient reverse genetics system for coronavirus.

Abstract excerpt

Avian infectious bronchitis virus (IBV), a Gamma coronavirus (γ-CoV), is one of the most economically important pathogens affecting the global poultry industry. In this study, we established a novel reverse genetics platform of infectious clone of GI-19 genotype IBV. This platform integrates low-copy plasmid vectors with Red/ET recombineering technology, combined with positive and counter-selection strategies,...

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• Chickens
• Coronavirus Infections
• Genotype
• Infectious bronchitis virus
• Plasmids