Engineering dimer mutants of human geranylgeranyl pyrophosphate synthase.

Abstract excerpt

Geranylgeranyl pyrophosphate synthase (GGPPS), a key enzyme in protein prenylation, plays a critical role in cellular signal transduction and is a promising target for cancer therapy. However, the enzyme's native hexameric quaternary structure presents challenges for crystallographic studies. The primary objective of this study was to engineer dimeric forms of human GGPPS to facilitate high-resolution...

Topics

• Humans
• Protein Multimerization
• Crystallography, X-Ray
• Protein Engineering
• Mutagenesis, Site-Directed
• Mutation
• Models, Molecular