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Article

Accurate identification of single-nucleotide variants in whole-genome-amplified single cells.

Nature methods - 1 May 2017

Dong Xiao, Zhang Lei, Milholland Brandon, Lee Moonsook, Maslov Alexander Y, Wang Tao, Vijg Jan

Abstract excerpt

Mutation analysis in single-cell genomes is prone to artifacts associated with cell lysis and whole-genome amplification. Here we addressed these issues by developing single-cell multiple displacement amplification (SCMDA) and a general-purpose single-cell-variant caller, SCcaller (https://github.com/biosinodx/SCcaller/). By comparing SCMDA-amplified single cells with unamplified clones from the same population,...

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Cells, Cultured
DNA Probes
Fibroblasts
Gene Library
Genome, Human

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nucleic Acid Amplification Techniques

Primary Cell Culture

Single-Cell Analysis

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Identifiers

PMID: 28319112
DOI: 10.1038/nmeth.4227
PMCID: PMC5408311

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