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Article

Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection.

Nucleic acids research - 1 Apr 2013

Liao Yiqun, Wang Xiaobo, Sha Chao, Xia Zhongmin, Huang Qiuying, Li Qingge

Abstract excerpt

Multiplex analytical systems that allow detection of multiple nucleic acid targets in one assay can provide rapid characterization of a sample while still saving cost and resources. However, few systems have proven to offer a solution for mid-plex (e.g. 10- to 50-plex) analysis that is high throughput and cost effective. Here we describe the combined use of fluorescence color and melting temperature (Tm) as a...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 23335787
DOI: 10.1093/nar/gkt004
PMCID: PMC3627564

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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