Article
A self-eliminating allelic-drive reverses insecticide resistance in Drosophila leaving no transgene in the population.
Nature communications - 17 Nov 2024
Auradkar Ankush, Corder Rodrigo M, Marshall John M, Bier Ethan
Abstract excerpt
Insecticide resistance (IR) poses a significant global challenge to public health and welfare. Here, we develop a locally-acting unitary self-eliminating allelic-drive system, inserted into the Drosophila melanogaster yellow (y) locus. The drive cassette encodes both Cas9 and a single gRNA to bias inheritance of the favored wild-type (1014 L) allele over the IR (1014 F) variant of the voltage-gated sodium ion...
Topics
- Animals
- Insecticide Resistance
- Alleles
- Drosophila melanogaster
- Transgenes
- Male
- Female
- CRISPR-Cas Systems
- Drosophila Proteins
- Animals, Genetically Modified
- Voltage-Gated Sodium Channels
- RNA, Guide, CRISPR-Cas Systems
- Gene Drive Technology