Article
An efficient C-glycoside production platform enabled by rationally tuning the chemoselectivity of glycosyltransferases.
Nature communications - 15 Oct 2024
Li Min, Zhou Yang, Wen Zexing, Ni Qian, Zhou Ziqin, Liu Yiling, Zhou Qiang, Jia Zongchao, Guo Bin, Ma Yuanhong, Chen Bo, Zhang Zhi-Min, Wang Jian-Bo
Abstract excerpt
Despite the broad potential applications of C-glycosides, facile synthetic methods remain scarce. Transforming glycosyltransferases with promiscuous or natural O-specific chemoselectivity to C-glycosyltransferases is challenging. Here, we employ rational directed evolution of the glycosyltransferase MiCGT to generate MiCGT-QDP and MiCGT-ATD mutants which either enhance C-glycosylation or switch to...
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