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Structure-guided mutations in CDRs for enhancing the affinity of neutralizing SARS-CoV-2 nanobody.

Biochemical and biophysical research communications - 19 Nov 2024

Singh Vishakha, Bhutkar Mandar, Choudhary Shweta, Nehul Sanketkumar, Kumar Rajesh, Singla Jitin, Kumar Pravindra, Tomar Shailly

Abstract excerpt

The optimization of antibodies to attain the desired levels of affinity and specificity holds great promise for the development of next generation therapeutics. This study delves into the refinement and engineering of complementarity-determining regions (CDRs) through in silico affinity maturation followed by binding validation using isothermal titration calorimetry (ITC) and pseudovirus-based neutralization...

Topics

Single-Domain Antibodies
SARS-CoV-2
Humans
Antibodies, Neutralizing
Spike Glycoprotein, Coronavirus
Mutation
Complementarity Determining Regions
Molecular Dynamics Simulation
Molecular Docking Simulation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Antibody Affinity

COVID-19

Antibodies, Viral

Protein Binding

Source

Identifiers

PMID: 39366179
DOI: 10.1016/j.bbrc.2024.150746

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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