Article
Engineering of tyrosyl tRNA synthetase.
Biochimie - 1 Jan 2000
Bedouelle H, Carter P, Waye M M, Winter G, Lowe D M, Wilkinson A J, Fersht A R
Abstract excerpt
The gene encoding the enzyme tyrosyl tRNA synthetase from Bacillus stearothermophilus has been systematically altered using synthetic oligonucleotides as mutagens. The construction of mutations has been facilitated by using strains of bacteria defective in mismatch repair and also by utilising a genetic marker in the M13 strain (such as an amber mutation, or an EcoK or EcoB site) which allows selection for the...
Topics
- Amino Acyl-tRNA Synthetases
- Binding Sites
- Calorimetry
- Escherichia coli
- Genes
- Genes, Bacterial
- Genetic Engineering
- Geobacillus stearothermophilus
- Hydrogen Bonding
- Kinetics
- Mutation
- RNA, Transfer