Article
A model of synthetase/transfer RNA interaction as deduced by protein engineering.
Nature - 1 Jan 2000
Bedouelle H, Winter G
Abstract excerpt
The recognition of transfer-RNA by their cognate aminoacyl-tRNA synthetases is the crucial step in the translation of the genetic code. In order to construct a structural model of the complex between the tyrosyl-tRNA synthetase (TyrTS) from Bacillus stearothermophilus and tRNATyr, 40 basic residues at the surface of the TyrTS dimer have been mutated by site-directed mutagenesis and heterodimers created in vitro...
Topics
- Amino Acyl-tRNA Synthetases
- Geobacillus stearothermophilus
- Kinetics
- Mutation
- Nucleic Acid Conformation
- Protein Binding
- Protein Conformation
- Protein Denaturation
- RNA, Transfer
- Structure-Activity Relationship
- Tyrosine