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Article

SIRT5 mutants reveal the role of conserved asparagine and glutamine residues in the NAD+-binding pocket.

FEBS letters - 1 Sept 2024

Yokoyama Takeshi, Takayama Yuki, Mizuguchi Mineyuki, Nabeshima Yuko, Kusaka Katsuhiro

Abstract excerpt

SIRT5, one of the mammalian sirtuins, specifically recognizes succinyl-lysine residues on proteins and catalyzes the desuccinylation reaction. In this study, we characterized SIRT5 mutants with hydrophobic amino acid substitutions at Q140 and N141, in addition to the catalytic residue H158, known as an active site residue, by the Michaelis-Menten analysis and X-ray crystallography. Kinetic analysis showed that...

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PMID: 39031546
DOI: 10.1002/1873-3468.14961

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

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