Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Two residues in the DNA binding site of Pif1 helicase are essential for nuclear functions but dispensable for mitochondrial respiratory growth.

Nucleic acids research - 24 Jun 2024

Gao Jun, Proffitt David R, Marecki John C, Protacio Reine U, Wahls Wayne P, Byrd Alicia K, Raney Kevin D

Abstract excerpt

Pif1 helicase functions in both the nucleus and mitochondria. Pif1 tightly couples ATP hydrolysis, single-stranded DNA translocation, and duplex DNA unwinding. We investigated two Pif1 variants (F723A and T464A) that have each lost one site of interaction of the protein with the DNA substrate. Both variants exhibit minor reductions in affinity for DNA and ATP hydrolysis but have impaired DNA unwinding activity....

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adenosine Triphosphate
Share to Cell Nucleus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Helicases

Share to DNA, Mitochondrial

Share to DNA, Single-Stranded

Share to Hydrolysis

Share to Mitochondria

Share to Saccharomyces cerevisiae Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 38752483
DOI: 10.1093/nar/gkae403
PMCID: PMC11194084

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Two residues in the DNA binding site of Pif1 helicase are essential for nuclear functions but dispensable for mitochondrial respiratory growth.PMID 38752483Read ↗

Saccharomyces cerevisiae MPH1 gene, required for homologous recombination-mediated mutation avoidance, encodes a 3' to 5' DNA helicase.Semantic proximity Read ↗

The role of XPB/Ssl2 dsDNA translocation processivity in transcription-start-site scanningSemantic proximity Read ↗

The Pif1 family helicase Pfh1 facilitates telomere replication and has an RPA-dependent role during telomere lengthening.Semantic proximity Read ↗

Identification of Saccharomyces cerevisiae Genes Whose Deletion Causes Synthetic Effects in Cells with Reduced Levels of the Nuclear Pif1 DNA Helicase.Semantic proximity Read ↗

The T4 phage SF1B helicase Dda is structurally optimized to perform DNA strand separation.Semantic proximity Read ↗

Irc3 is a monomeric DNA branch point-binding helicase in mitochondria of the yeast Saccharomyces cerevisiae.Semantic proximity Read ↗

Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase.Semantic proximity Read ↗

The N-terminal domain of the human mitochondrial helicase Twinkle has DNA binding activity crucial for supporting processive DNA synthesis by Polymerase γSemantic proximity Read ↗

The fission yeast pfh1(+) gene encodes an essential 5' to 3' DNA helicase required for the completion of S-phase.Semantic proximity Read ↗

A Critical Role for Dna2 at Unwound Telomeres.Semantic proximity Read ↗

Alternative translation initiation by ribosomal leaky scanning produces multiple isoforms of the Pif1 helicase.Semantic proximity Read ↗

Evidence suggesting that Pif1 helicase functions in DNA replication with the Dna2 helicase/nuclease and DNA polymerase delta.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.