Article
Mutational dissection of a hole hopping route in a lytic polysaccharide monooxygenase (LPMO).
Nature communications - 10 May 2024
Ayuso-Fernández Iván, Emrich-Mills Tom Z, Haak Julia, Golten Ole, Hall Kelsi R, Schwaiger Lorenz, Moe Trond S, Stepnov Anton A, Ludwig Roland, Cutsail Iii George E, Sørlie Morten, Kjendseth Røhr Åsmund, Eijsink Vincent G H
Abstract excerpt
Oxidoreductases have evolved tyrosine/tryptophan pathways that channel highly oxidizing holes away from the active site to avoid damage. Here we dissect such a pathway in a bacterial LPMO, member of a widespread family of C-H bond activating enzymes with outstanding industrial potential. We show that a strictly conserved tryptophan is critical for radical formation and hole transference and that holes traverse...
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