Article
Massively parallel dissection of RNA in RNA-protein interactions in vivo.
Nucleic acids research - 10 Jun 2024
Lee Yu Hsuan, Hass Evan P, Campodonico Will, Lee Yong Kyu, Lasda Erika, Shah Jaynish S, Rinn John L, Hwang Taeyoung
Abstract excerpt
Many of the biological functions performed by RNA are mediated by RNA-binding proteins (RBPs), and understanding the molecular basis of these interactions is fundamental to biology. Here, we present massively parallel RNA assay combined with immunoprecipitation (MPRNA-IP) for in vivo high-throughput dissection of RNA-protein interactions and describe statistical models for identifying RNA domains and parsing the...
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