Article

Individual nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) to determine protein-RNA interactions

2017-02-14

Abstract excerpt

RNA-binding proteins (RBPs) interact with and determine the fate of many cellular RNA transcripts. In doing so they help direct many essential roles in cellular physiology, whilst their perturbed activity can contribute to disease aetiology. In this chapter we detail a functional genomics approach, termed individual nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP), that can determine the inte...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 1a90586c-0c99-534a-8f64-38ac8c1a3d29
DOI: 10.1101/108118

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Individual nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) to determine protein-RNA interactionsDOI 10.1101/108118Read

Comparative analysis of LIN28-RNA binding sites identified at single nucleotide resolution.Semantic proximity Read

PAR-CLIP and streamlined small RNA cDNA library preparation protocol for the identification of RNA binding protein target sites.Semantic proximity Read

RBP-Tar – a searchable database for experimental RBP binding sitesSemantic proximity Read

uvCLAP: a fast, non-radioactive method to identify <i>in vivo</i> targets of RNA-binding proteinsSemantic proximity Read

dirCLIP profiles variant-specific RNA-protein interactions via nanopore long-read sequencingSemantic proximity Read

Resources for the comprehensive discovery of functional RNA elementsSemantic proximity Read

The Human RNA-Binding Proteome and Its Dynamics During Arsenite-Induced Translational ArrestSemantic proximity Read

Comparing Experimental and Computational Approaches to Identify RNA-Protein InteractionsSemantic proximity Read

Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome.Semantic proximity Read

Massively Parallel Dissection of RNA in RNA-protein interactions <i>in vivo</i>Semantic proximity Read

Low-Dose Ionizing Radiation-Crosslinking Immunoprecipitation (LDIR-CLIP) Identified Irradiation-Sensitive RNAs for RNA-Binding Protein HuR-Mediated DecaySemantic proximity Read

Mapping the Human Proteome with Physical Access to DNASemantic proximity Read