Article
Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization specificity.
Genetic analysis, techniques and applications - 1 Jun 1990
Kajimura Y, Krull J, Miyakoshi S, Itakura K, Toyoda H
Abstract excerpt
Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cell Line
- DNA
- HLA-DQ Antigens
- HLA-DR Antigens
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Sequence Homology, Nucleic Acid
- Temperature