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Optimization of seeding density of OP9 cells to improve hematopoietic differentiation efficiency

25 Mar 2024

Abstract excerpt

Abstract Background OP9 mouse stromal cell line has been widely used to induce differentiation of human embryonic stem cells (hESCs) into hematopoietic stem/progenitor cells (HSPCs). However, the whole co-culture procedure usually needs 14–18 days, including preparing OP9 cells at least 4 days. Therefore, the inefficient differentiation system is not appreciated. We aimed to optimize the culture conditions to...

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PMID: 38523262
DOI: 10.1186/s12860-024-00503-x

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