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Human Adult Marrow Cells Support Prolonged Expansion of Human Embryonic Stem Cells in Culture

1 Mar 2003

Abstract excerpt

Prolonged propagation of human embryonic stem (hES) cells is currently achieved by coculture with primary mouse embryonic fibroblasts (MEFs) serving as feeder cells. Unlike mouse ES cells, adding growth factors such as leukemia inhibitory factor is insufficient to maintain undifferentiated hES cells without feeder cells. The presence of uncharacterized rodent cells or crude extracts imposes a risk to the clinical...

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PMID: 12634409
DOI: 10.1634/stemcells.21-2-131

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