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Article

Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas - 15 Mar 2018

Chen Haide, Jiang Mengmeng, Xiao Lei, Huang He

Abstract excerpt

Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34+ cells from different stages of differentiation. According to the dynamic gene expression of hematopoietic transcription factors, we...

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Identifiers

PMID: 29561959
DOI: 10.1590/1414-431X20187183
PMCID: PMC5875903

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ERG

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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