Free energy perturbations in enzyme kinetic models reveal cryptic epistasis.

Abstract excerpt

Epistasis-the context-dependence of mutational effects-is a key driver of protein evolution, influencing adaptive pathways and functional diversity. While specific epistasis arises from direct physical interactions between mutations, non-specific epistasis emerges when a non-linear mapping links a protein's biophysical properties to its function. Enzyme kinetic parameters map directly to free energies, enabling...

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