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Article

Site-directed mutagenesis shows that tyrosine 248 of carboxypeptidase A does not play a crucial role in catalysis.

Nature - 1 Jan 2000

Gardell S J, Craik C S, Hilvert D, Urdea M S, Rutter W J

Abstract excerpt

The residue Tyr 248 of carboxypeptidase A (CPA) is thought to play a role in catalysis by contributing a proton to the incipient amine anion generated during cleavage of peptide substrates. To test this hypothesis we have modified the rat CPA cDNA by site-directed mutagenesis so that the codon for Tyr 248 is replaced by that for Phe. Here, we report the expression of the cDNAs for proCPA and its Tyr-to-Phe...

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PMID: 3840231
DOI: 10.1038/317551a0

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