Article
Delineation of the catalytic core of phenylalanine hydroxylase and identification of glutamate 286 as a critical residue for pterin function.
The Journal of biological chemistry - 12 Aug 1994
Dickson P W, Jennings I G, Cotton R G
Abstract excerpt
Rat phenylalanine hydroxylase was expressed in Escherichia coli. High level expression was achieved when the transformed E. coli were incubated at 27 degrees C for 24 h. A series of truncated fragments were expressed. The smallest fragment that gave an active soluble protein was from Leu142 to Phe410. This fragment corresponds closely to the region where there is highest homology between the three aromatic amino...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Catalysis
- Circular Dichroism
- Cloning, Molecular
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Glutamates
- Glutamic Acid