The establishment of a multiplex fluorescent polymerase chain reaction coupled with capillary electrophoresis analysis technology enables the simultaneous detection of 16 genotypes of human papillomavirus.

Abstract excerpt

BACKGROUND: The detection and accurate genotyping of human papillomavirus (HPV) infection is critical for preventing and effectively treating cervical cancer. METHODS: A multiplex fluorescent polymerase chain reaction (PCR) coupled with a capillary electrophoresis method was developed for the simultaneous detection of the 16 most prevalent HPV genotypes. Twenty-five pairs of primers were ultimately selected to...

Topics

• Female
• Humans
• Human Papillomavirus Viruses
• Papillomavirus Infections
• Sensitivity and Specificity
• Multiplex Polymerase Chain Reaction
• Genotype
• Uterine Cervical Neoplasms
• Electrophoresis, Capillary