Article
Comparison of multiplex PCR capillary electrophoresis assay and PCR-reverse dot blot assay for human papillomavirus DNA genotyping detection in cervical cancer tissue specimens.
Frontiers in public health - 1 Jan 2024
Qin Lei, Li Dan, Wang Zhihui, Lan Jianyun, Han Chunrong, Mei Jing, Geng Jianxiang
Abstract excerpt
Background: The study aimed to evaluate the positivity rates and genotype distribution of the multiplex PCR capillary electrophoresis (MPCE) and PCR-Reverse Dot Blot (PCR-RDB) assays for human papillomavirus (HPV) detection in cervical cancer tissue specimens, and to explore their detection principles and applications in large-scale population screening. Methods: The MPCE and PCR-RDB assays were performed...
Topics
- Humans
- Female
- Electrophoresis, Capillary
- Uterine Cervical Neoplasms
- Middle Aged
- Multiplex Polymerase Chain Reaction
- Genotype
- Adult
- Papillomaviridae
- DNA, Viral
- Papillomavirus Infections
- Genotyping Techniques
- Aged