Article
Multiplex PCR assay for the rapid identification of human papillomavirus genotypes 16, 18, 45, 35, 66, 33, 51, 58, and 31 in clinical samples.
Archives of virology - 1 Jan 2015
Tsakogiannis D, Diamantidou V, Toska E, Kyriakopoulou Z, Dimitriou T G, Ruether I G A, Gortsilas P, Markoulatos P
Abstract excerpt
The causal association between persistent human papillomavirus (HPV) infection and cervical cancer has lead to the development of a variety of molecular assays for HPV detection. The present study focused on the development of a simple, sensitive and cost-effective HPV genotyping method based on multiplex PCR methodology that could be easily performed in small laboratories. Three multiplex PCR assays were...
Topics
- DNA, Viral
- Genotype
- Humans
- Multiplex Polymerase Chain Reaction
- Papillomaviridae
- Plasmids
- Sensitivity and Specificity