Genotyping of human papillomaviruses by a novel one-step typing method with multiplex PCR and clinical applications.

doi:10.1128/JCM.00793-07

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Article

Genotyping of human papillomaviruses by a novel one-step typing method with multiplex PCR and clinical applications.

Journal of clinical microbiology - 1 Apr 2008

Nishiwaki Morie, Yamamoto Tomohiro, Tone Somako, Murai Taichi, Ohkawara Tatsuya, Matsunami Takakuni, Koizumi Motoiki, Takagi Yoshitake, Yamaguchi Jun, Kondo Nobuo, Nishihira Jun, Horikawa Takeharu, Yoshiki Takashi

Abstract excerpt

We describe here a rapid, high-throughput genotyping procedure that allows the simultaneous detection of 16 high- and low-risk genital human papillomavirus (HPV) types by multiplex PCR in a single reaction tube. Multiplex PCR is based on the amplification of HPV DNA by sets of HPV genotype-specific primers, and the genotypes of HPV are visually identified by the sizes of amplicons after they are separated by...

Topics

Adolescent
Adult
Aged
Aged, 80 and over
Cervix Uteri
DNA Primers
Electrophoresis, Capillary
Female
Genotype
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Middle Aged

Papillomaviridae

Papillomavirus Infections

Polymerase Chain Reaction

Prevalence

Sensitivity and Specificity

Uterine Cervical Neoplasms

Uterine Cervical Dysplasia

Source

Open in PubMed Open full text

Identifiers

PMID: 18234872
DOI: 10.1128/JCM.00793-07
PMCID: PMC2292906

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-18234872-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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