Article
Robust nontarget DNA-triggered catalytic hairpin assembly amplification strategy for the improved sensing of microRNA in complex biological matrices.
The Analyst - 20 Nov 2023
Yang Ruining, Liu Xingfen, Hu Junbo, Xu Hui, Song Jixiang, Zhou Huiyu, Li Meixing, Huang Yanqin, Zhang Lei, Fan Quli
Abstract excerpt
A simple but robust fluorescence strategy based on a nontarget DNA-triggered catalytic hairpin assembly (CHA) was constructed to probe microRNA-21 (miR-21). A short ssDNA rather than degradable target miRNA was employed as an initiator. Two molecular beacons needed to assist the CHA process were simplified to avoid unfavorable nonspecific interactions. In the presence of the target, the initiator was released...
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