Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene.

doi:10.1038/324034a0

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Article

Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene.

Nature - 1 Jan 2000

Thomas K R, Capecchi M R

Abstract excerpt

Injection of homologous DNA sequences into nuclei of cultured mammalian cells induces mutations in the cognate chromosomal gene. It appears that these mutations result from incorrect repair of a heteroduplex formed between the introduced and the chromosomal sequence. This phenomenon is termed 'he...

Topics

Animals
Cell Line
DNA, Recombinant
Drug Resistance, Microbial
Fibroblasts
Mice
Models, Genetic
Mutation
Neomycin
Plasmids
Protein Biosynthesis
Sequence Homology, Nucleic Acid
Transfection

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