Article
Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene.
Nature - 1 Jan 2000
Thomas K R, Capecchi M R
Abstract excerpt
Injection of homologous DNA sequences into nuclei of cultured mammalian cells induces mutations in the cognate chromosomal gene. It appears that these mutations result from incorrect repair of a heteroduplex formed between the introduced and the chromosomal sequence. This phenomenon is termed 'heteroduplex induced mutagenesis'. The high frequency of these events suggests that this method may prove useful for...
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