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Single Amino Acid Mutation Decouples Photochemistry of the BLUF Domain from the Enzymatic Function of OaPAC and Drives the Enzyme to a Switched-on State.

Journal of molecular biology - 1 Mar 2024

Tolentino Collado Jinnette, Bodis Emoke, Pasitka Jonatan, Szucs Mihaly, Fekete Zsuzsanna, Kis-Bicskei Nikolett, Telek Elek, Pozsonyi Kinga, Kapetanaki Sofia M, Greetham Greg, Tonge Peter J, Meech Stephen R, Lukacs Andras

Abstract excerpt

Photoactivated adenylate cyclases (PACs) are light-activated enzymes that combine a BLUF (blue-light using flavin) domain and an adenylate cyclase domain that are able to increase the levels of the important second messenger cAMP (cyclic adenosine monophosphate) upon blue-light excitation. The light-induced changes in the BLUF domain are transduced to the adenylate cyclase domain via a mechanism that has not yet...

Topics

Adenylyl Cyclases
Bacterial Proteins
Flavins
Light
Mutation
Glutamine
Protein Domains
Electron Transport
Enzyme Activation
Oscillatoria

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Identifiers

PMID: 37827329
DOI: 10.1016/j.jmb.2023.168312
PMCID: PMC11256462

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