Article
Photoconvertible fluorescent protein EosFP: biophysical properties and cell biology applications.
Photochemistry and photobiology - 1 Jan 2000
Nienhaus G Ulrich, Nienhaus Karin, Hölzle Angela, Ivanchenko Sergey, Renzi Fabiana, Oswald Franz, Wolff Michael, Schmitt Florian, Röcker Carlheinz, Vallone Beatrice, Weidemann Wolfgang, Heilker Ralf, Nar Herbert, Wiedenmann Jörg
Abstract excerpt
EosFP is a fluorescent protein from the coral Lobophyllia hemprichii that changes its fluorescence emission from green to red upon irradiation with near-UV light. Here we present the spectroscopic properties of wild-type EosFP and a variety of monomeric and dimeric mutants and provide a structural interpretation of its oligomerization and photoconversion, which is based on X-ray structure analysis of the green...
Topics
- Amino Acids
- Androgens
- Animals
- Anthozoa
- Biotechnology
- Cell Physiological Phenomena
- Crystallography, X-Ray
- Dimerization
- Luminescent Proteins
- Mutation
