Article
Site-directed RNA editing by adenosine deaminase acting on RNA for correction of the genetic code in gene therapy.
Gene therapy - 1 Dec 2017
Azad Md T A, Bhakta S, Tsukahara T
Abstract excerpt
Site-directed RNA editing is an important technique for correcting gene sequences and ultimately tuning protein function. In this study, we engineered the deaminase domain of adenosine deaminase acting on RNA (ADAR1) and the MS2 system to target-specific adenosines, with the goal of correcting G-to-A mutations at the RNA level. For this purpose, the ADAR1 deaminase domain was fused downstream of the RNA-binding...
Topics
- Adenosine
- Adenosine Deaminase
- Alleles
- Blotting, Western
- Codon, Terminator
- Genetic Code
- Genetic Therapy
- Green Fluorescent Proteins
- Humans
- Inosine
- Point Mutation
- RNA Editing