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Real-Time PCR High-Resolution Melting Assays for the Detection of Foodborne Pathogens.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Singh Prashant, Velez Frank J

Abstract excerpt

Real-time PCR high-resolution melting assays are a method for the identification of single nucleotide polymorphisms (SNPs). The assay is performed by amplifying a short DNA fragment using a specific primer pair flanking a target SNP in the presence of a high-resolution melting dye. The HRM analysis of amplicons groups the samples based on the differences in the melting temperature and the shape of the melt...

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PMID: 37608101
DOI: 10.1007/978-1-0716-3358-8_4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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