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Article

A novel high-resolution melting analysis-based method for Salmonella genotyping.

Journal of microbiological methods - 1 May 2020

Hu Miaomiao, Yang Dong, Wu Xiaoli, Luo Meng, Xu Feng

Abstract excerpt

To establish a simple and rapid high-resolution melting curve (HRM) method, 5 different strains of Salmonella were identified by adding DNA denaturants at different concentrations into the HRM system to change the characteristics of DNA melting and to obtain different Tm (dissolving temperature) values of DNA from different target bacteria. When the concentration of n-butanol was 7% (v/v), the Tm value of the...

Topics

Bacterial Typing Techniques
DNA, Bacterial
Food Safety
Genotype
Genotyping Techniques
Nucleic Acid Denaturation
Polymerase Chain Reaction
RNA, Ribosomal, 16S
Salmonella
Salmonella Infections
Sensitivity and Specificity

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Temperature

Transition Temperature

Source

Identifiers

PMID: 31837350
DOI: 10.1016/j.mimet.2019.105806

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