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Development of High Resolution Melting Analysis for the Diagnosis of Human Malaria

28 Oct 2015

Abstract excerpt

Molecular detection has overcome limitations of microscopic examination by providing greater sensitivity and specificity in Plasmodium species detection. The objective of the present study was to develop a quantitative real-time polymerase chain reaction coupled with high-resolution melting (qRT-PCR-HRM) assay for rapid, accurate and simultaneous detection of all five human Plasmodium spp. A pair of primers...

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PMID: 26507008
DOI: 10.1038/srep15671

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