Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Genotyped functional screening of soluble Fab clones enables in-depth analysis of mutation effects.

Scientific reports - 11 Aug 2023

Oksanen Sami, Saarinen Roope, Korkiakoski Anttoni, Lamminmäki Urpo, Huovinen Tuomas

Abstract excerpt

Monoclonal antibodies (mAbs) and their fragments are widely used in therapeutics, diagnostics and basic research. Although display methods such as phage display offer high-throughput, affinities of individual antibodies need to be accurately measured in soluble format. We have developed a screening platform capable of providing genotyped functional data from a total of 9216 soluble, individual antigen binding...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antibodies, Monoclonal
Share to Immunoglobulin Fab Fragments

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutation

Share to Peptide Library

Source

Open in PubMed Open full text

Identifiers

PMID: 37567990
DOI: 10.1038/s41598-023-40241-2
PMCID: PMC10421887

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Genotyped functional screening of soluble Fab clones enables in-depth analysis of mutation effects.PMID 37567990Read ↗

Position-Specific Enrichment Ratio Matrix scores predict antibody variant properties from deep sequencing dataSemantic proximity Read ↗

Position-Specific Enrichment Ratio Matrix scores predict antibody variant properties from deep sequencing dataSemantic proximity Read ↗

Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodiesSemantic proximity Read ↗

An integrated technology for quantitative wide mutational scanning of human antibody Fab libraries.Semantic proximity Read ↗

An integrated technology for quantitative wide mutational scanning of human antibody Fab librariesSemantic proximity Read ↗

Discovering Selected Antibodies From Deep-Sequenced Phage-Display Antibody Library Using ATTILASemantic proximity Read ↗

Multiplexed, multimodal profiling of the intracellular activity, interactions, and druggability of protein variants using LABEL-seqSemantic proximity Read ↗

An end-to-end automated platform process for high-throughput engineering of next-generation multi-specific antibody therapeuticsSemantic proximity Read ↗

Optimizing antibody affinity and stability by the automated design of the variable light-heavy chain interfacesSemantic proximity Read ↗

Deep mutational scanning of an antibody against epidermal growth factor receptor using mammalian cell display and massively parallel pyrosequencingSemantic proximity Read ↗

Improving genotyped functional screening: A versatile closed-tube PCR for Illumina library generation reduces background sequences in multiplexed sequencing.Semantic proximity Read ↗

mmCSM-AB: guiding rational antibody engineering through multiple point mutationsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.